TA cloning vector

kuysy at waikato.ac.nz kuysy at waikato.ac.nz
Wed Aug 4 21:32:33 EST 1993


In article <CB7FBL.HwL at athena.cs.uga.edu>, ALVAREZ at mgif.genetics.uga.edu (CARLOS ALVAREZ) writes:
> In <1993Aug3.205333.24726 at galileo.cc.rochester.edu> PHIP at BPHVAX.BIOPHYSICS.ROCHESTER.EDU writes:
> 
>> Hello, net, 
>> 
>> I'll be doing some cloning of PCR products in the near future and I was 
>> planning on using TA cloning as described by Invitrogen.  I was hoping, 
>> however, not to have to buy their kit.  Does anyone know of a vector 
>> that is easily obtained and can be used for this purpose (i.e., can be 
>> cut with an enzyme which leaves a single T overhang).  Thanks in advance,
>> I'll post a summary later.
>> 
>> Rick Willis 
>> University of Rochester Cancer Center
>> 
> 
> Check out the pT7Blue T-Vector Introductory Kit from Novagen (800-526-7319).
> For $75 you get vector, competent cells, SOC medium, positive control and
> protocols enough for 10 ligations. In our hands it has worked very well
> every time we have used to clone PCR products.
> Good Luck
> Carlos
> PS. We do not have any bussiness relationship with Novagen.

You could try the vector pDK101  (kovalic et al NAR 19: 4560) . It is pgem
based and uses XCM1 to leave t overhangs.   Phone Bernie Wiesblum 608-262-0972.



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