In article <22l69e$r1b at huon.itd.adelaide.edu.au>, rmorona at microb.adelaide.edu.au writes:
>> I am interested in speeding up our traditional Southern blotting methods. I would appreciated
> receiving any un-biased opinions on this subject, rather than the info presented by sales
> representatives. Does anyone have experience with vacuum blotting versus electroblotting?
>> Thanks very much.
>>> Renato Morona
> Department of Microbiology
> and Immunology
> University of Adelaide
> Adelaide, South Australia
> EMAIL ADDRESS: RMORONA at MICROB.ADELAIDE.EDU.AU>For convenience try the method in Biotechniques Vol 10 No.3 1991 Pg 334.
Basically it soaks the gel beforehand (acid then alkali) and dispenses with
the wicks and buffer tanks. (3hrs to overnight work fine)>
Regards Adrian Molenaar (Alias YM Kuys)