protein expression systems

Alberto I Roca roca at macc.wisc.edu
Thu Aug 5 20:00:46 EST 1993


paul,
regarding your possibly unstable protein in e.coli, have you tried any
protease deficient strains? i know of a couple:
BL21 from Novagen. lon(minus) and ompT(minus)
CAG626 lon(minus)
CAG597 rpoH(minus)
CAG629 lon(minus) rpoH(minus)
for the CAG strains see Baker PNAS 81(21):6779(84) for a description.

unfortunately they haven't worked for my particular protein, but i
thought i
would pass on the information.

alberto :|
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Alberto I. Roca                 Internet: roca at macc.wisc.edu
Biochemistry                    Bitnet:   roca at wiscmacc
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University of Wisconsin-Madison
Madison, Wisconsin 53706 USA



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