DNA labeling

Sylvie Croteau croteau at jouy.inra.fr
Fri Aug 6 06:20:31 EST 1993


Hi netters, 

				I would like to label nucleotides with T4 polynucleotide kinase but I
am not allowed to use 32-P.
			 Does anyone know if it is possible to use Adenosine 5' [gamma-35 S]
thiotriphosphate instead of Adenosine 5' [gammag-32 P] triphosphate ?
Are there any special conditions to allow the enzyme to use this substrate
?

			Thanks !

Sylvie CROTEAU
Laboratoire de Biologie Applique
INSA-Bat 406
20, avenue Eisntein
69621 VILLEURBANNE CEDEX
FRANCE

e-mail : croteau at jouy.inra.fr



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