This is the reference to which I was refering in my previous post regarding
ultrasonic ligations, in 15 minutes!
J. B Cooper, D. H. Halverson and C. Coldren
NAR 7, Vol. 21 (April 7, 1993), p1682
"Ultrasonic Ligation for Rapid High Efficiency Subcloning in Plamsid Vectors"
Basically put standard ligation in ultrasonic cleaning bath, wait 15', done!!
Any comments appreciated!