In article <1993Aug8.155348.20516 at infodev.cam.ac.uk> arosenth at crc.ac.uk (Dr. A. Rosenthal) writes:
>>This is the reference to which I was refering in my previous post regarding
>ultrasonic ligations, in 15 minutes!
>>J. B Cooper, D. H. Halverson and C. Coldren
>NAR 7, Vol. 21 (April 7, 1993), p1682
>"Ultrasonic Ligation for Rapid High Efficiency Subcloning in Plamsid Vectors"
>>Basically put standard ligation in ultrasonic cleaning bath, wait 15', done!!
>>Any comments appreciated!
Uh, Am I missing something here? I put my ligations on the bench for 5min
and they are done.