Ultra Fast Ligations */Refs/*
jgraham at bronze.ucs.indiana.edu
Mon Aug 9 11:28:05 EST 1993
> Uh, Am I missing something here? I put my ligations on the bench for 5min
> and they are done.
Yes. Efficiency. The authors claim equal results with 15 min with sonication
and overnight ligation, both at 16C. Two hours at room temp gave about
3-fold less transformants. Amazingly, no indication of the sonication
frequency or intensity is given.
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