Ultrasonic Ligation

Dr. Duncan Clark Duncan at genesys.demon.co.uk
Tue Aug 10 14:45:07 EST 1993


Hi All,

I tried the NAR method using a small Ultrasonic cleaning bath (Jencons
Litd., UK, 80W, 40kHz). I tried ligating 1ug EcoR V cut lambda for blunt and
1ug Hind III lambda for sticky ended ligation. In a 15 minute sonication at 16C
no ligation was apparent with the blunt and only slight with the sticky ie
no more than non-sonicated controls. I was not impressed and haven't tried
it again. Maybe my cleaning bath was not the right sort. 

Duncan Clark
-- 
-----------------------------------------------------------------------------
 Duncan Clark                       | Internet:   duncan at genesys@demon.co.uk
 GeneSys Ltd.                       | Compuserve: 100015.1406 at compuserve.com
-----------------------------------------------------------------------------



More information about the Methods mailing list