In article <1993Aug10.175048.1 at molbiol.ox.ac.uk> mwgaunt at molbiol.ox.ac.uk writes:
> The company 5'-> 3 Prime ( address by Graham Atherton, 5 Aug 1993)
> catagorises its gel barriers, which it terms phase lock gels, as heavy
> and light depending on the density. The two catagories are used for
> different manipulations, for instance high is always used on RNA
> extractions ( psst company seems eager to give free samples).
>> Hope this helps
> Mike Gaunt (mwgaunt at ac.ox.uk.)
>> Inst. Virology
> Oxford UK.
Usually I avoid phenol whenever I can, but when extracting DNA from
methanogens, I have to phenol extract it to get it to cut. SInce I started
using Phase Lock gel tubes (from 5' -> 3'), my DNA yield has increased
substantially from not leaving half of the sample in the tube to avoid
capturing the interface. I use the 15 ml centrifuge tube and Phase Lock
Heavy for this.
The other time I use Phase Lock (Light) gel is when phenol extracting after
primer extension of bacterial RNA.
Both work well... usual disclaimers apply.
John Nash | Email: Nash at biologysx.lan.nrc.ca.
Institute for Biological Sciences |
National Research Council of Canada | Email to my other NRC accounts
Ottawa, Ontario, Canada. | is usually forwarded here.
*** Disclaimer: All opinions are mine, not NRC's! ***