In article <2575 at rc1.vub.ac.be>, dacoo at is2e.vub.ac.be (Coornaert D.) writes:
> Hum Hum,
> I find strange that sonication would increase the ligation rate
> Sonication is most often use for breaking DNA.
> That the authors do not answer, make me think that this was
> a first April joke .
>> May the Ligase be with you !
>>Well, there is hardly anybody who would not be surprised I guess, but my
>theory is along
>the lines, that ultrasound enhances the collision frequency of the DNA
>therefore the apparent concentration, which would explain the mechanism.
>Of course the frequency of ultrasound used in a sonicator is higher than
>that in the
>cleaning baths used in the ref. I gave, leading to DNA breakage due to
Does anyone have an idea of what the upper limit (if any) of the size of DNA
one can use is?
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