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Electroporating with lambda

GIETZ at bldghsc.lan1.umanitoba.ca GIETZ at bldghsc.lan1.umanitoba.ca
Tue Aug 10 13:07:00 EST 1993

----------------------BSS032 at BANGOR.AC.UK Writes------------------------
Hi Netters,

Has anyone tried electroporation as an alternative to in vitro packaging
in order to make a lambda library? Comments, experiences, references welcomed.
Jon R Sayers (Cell and Molecular Biology), Biochemistry Dept.,
University College North Wales, Univ of Wales, Bangor, LL57 2UW.
Tel +44 248 38 23 54
Fax +44 248 370731
Email BSS032 at BANGOR.AC.UK

    I am in the process of doing that experiment. I'll tell you what
happens tomorrow!  I'll be electroporating a ligation but it will give some
idea how it works.
Dan Gietz
R.Daniel Gietz Ph.D.
Assistant Professor
Department of Human Genetics
University of Manitoba
770 Bannatyne Ave, Rm 250
Winnipeg, Manitoba, Canada
R3E 0W3
Tel.: (204)789-3458
Fax.: (204)786-8712
"Trying to do the Manitoba Thing"

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