Kits for cDNA Making
VAN BELZEN at PA1
belzen at pa1.fgg.eur.nl
Wed Aug 11 10:13:44 EST 1993
In article <1993Aug9.225447.19989 at fcom.cc.utah.edu> Tom Doak <
herricklab at bioscience.utah.edu> writes:>From: Tom Doak <herricklab@
bioscience.utah.edu>>Subject: Kits for cDNA Making
>Date: Mon, 9 Aug 93 22:54:47 GMT
>We used a Perkin-Elmer cDNA kit a couple years ago, and weren't very
>impressed with what we got, though perhaps it was a problem of our RNA.
>Does anyone have comments on the current commercially available kits.
>herricklab at bioscience.utah.edu
We have constructed two cDNA libraries with the Stratagene lambda ZAP
directional cDNA synthesis kit. The best library contained 7.5*10^6 pfu/ug
RNA, the second perhaps half of that which is still satisfactory...
Nico van Belzen (belzen at pa1.fgg.eur.nl)
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