Ultrasonic Ligation

Dr. A. Rosenthal arosenth at crc.ac.uk
Wed Aug 11 10:19:37 EST 1993


In article <745011907snz at genesys.demon.co.uk>, Duncan at genesys.demon.co.uk ("Dr. Duncan Clark") writes:
> Hi All,
> 
> I tried the NAR method using a small Ultrasonic cleaning bath (Jencons
> Litd., UK, 80W, 40kHz). I tried ligating 1ug EcoR V cut lambda for blunt and
> 1ug Hind III lambda for sticky ended ligation. In a 15 minute sonication at 16C
> no ligation was apparent with the blunt and only slight with the sticky ie
> no more than non-sonicated controls. I was not impressed and haven't tried
> it again. Maybe my cleaning bath was not the right sort. 
> 
> Duncan Clark

Duncan, although it may seem obvious, may I ask whether you tried to transform the same ligation reaction
after standard o/n ligation at 16`C for comparison. IMHO this would be the ultimate proof for failure
or success.



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