Ultrasonic Ligation

James Gibbs gibbs at husc4.harvard.edu
Thu Aug 12 16:24:01 EST 1993

gibbs at husc4.harvard.edu (James Gibbs) writes:

>For those interested in ultrafast ligations, I would strongly recommend
>the ligation kit made by TaKaRa. It only takes 15 minutes at 16C or RT. I
>called the company to ask how it works and they told me it is T4 ligase
>with a polyionic substance like dextran to increase local DNA end
>concentrations. The efficiency is superb in our hands.

>Sorry I don't have a phone number or address handy, as I am home now (well,
>not when you're reading this, but when I wrote it :) ).

>gibbs at husc.harvard.edu

The TaKaRa DNA ligation kit can be purchased from:
PanVera Corp.
565 Science Drive
Madison, WI 53711
gibbs at husc.harvard.edu

gibbs at husc.harvard.edu

More information about the Methods mailing list