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How do you make a giant oligo?

George W Chacko gchacko at magnus.acs.ohio-state.edu
Thu Aug 12 16:23:34 EST 1993


In article <robison1.745185662 at husc.harvard.edu> robison1 at husc10.harvard.edu (Keith Robison) writes:
>There should be a number of references on this subject out there.
>The two "classic" examples are Khorana's synthesis of tRNA in the
>early 70's and the Boyer group's synthesis of a growth hormone gene
>in the late 70's.  If it worked all the way back then, it should work
>now :-)

While on the subject of classic examples the synthesis of a gene for
bovine rhdopsin also from Khorana's group comes to mind. 

Total Synthesis Of A Gene For Bovine Rhodopsin.
Ferretti et al. PNAS. 83:599-603. (1986)

They ligated 72 oligos to generate a fragment 1072 base pairs long.

George



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