Formamide extraction of lambda?
Helgi Briem Magnusson
hbriem at rhi.hi.is
Fri Aug 13 09:37:37 EST 1993
After running a lambda lysate on a CsCl gradient I was about
to extract the DNA using our usual method, dialysis,
3x phenol and chloroform extractions and dialysis again,
when I came across an alternate protocol in Ausubel's Red Book
section 1.13.3. This involves adding very strong Tris/EDTA and
formamide, letting it sit for half an hour, then adding EtOH,
quick spin and wash with 70% EtOH. This method seems so easy
that I figure there must be a catch to it. Has anyone out
there tried this formamide protocol and are they happy with it?
Are there any problems with it? How about yield?
Inst. of Exp. Path.
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