Guanidin-thiocyanat acid phenol vs. CsCl centrifugation in RNA
Has anyone observed that large or small transcripts are selectively
removed by using one of these methods.
I have observed, that small RNAs as tRNA are removed during CsCl
centrifugation, but not when using the guanidin thiocyanat-acid phenol
Has anyone observed the same, and is it possible that the reverse
is possible, that very large RNAs are removed during acid-phenol but not
in CsCl centrifugation.
Please reply at:
nelleman at biobase.aau.dk