RNA purification

Lars Nellemann nelleman at biobase.aau.dk
Fri Aug 13 04:18:09 EST 1993


Guanidin-thiocyanat acid phenol vs. CsCl centrifugation in RNA
purification.

Has anyone observed that large or small transcripts are selectively 
removed by using one of these methods.

I have observed, that small RNAs as tRNA are removed during CsCl
centrifugation, but not when using the guanidin thiocyanat-acid phenol
purification.
Has anyone observed the same, and is it possible that the reverse
is possible, that very large RNAs are removed during acid-phenol but not 
in CsCl centrifugation.

Please reply at:

nelleman at biobase.aau.dk
 
 



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