DNA cleavage in a RNA/DNA mixed oligo ?

vonahsen at orchid.UCSC.EDU vonahsen at orchid.UCSC.EDU
Mon Aug 16 12:16:42 EST 1993


We synthesized a mixed DNA/RNA oligo on the machine. I 5' endlabeled
the oligo after purification and could easily determine the
positions where I have RNA either by alkaline hydrolysis or T2 digest.
Question: Does anybody know of a similar method to determine the
positions where I got DNA ? DNAse I does not work,I guess it needs
the B-form. The oligo is probably folded partially into a helix
and partially single-stranded.

Thanks,
Uwe

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uwe at fangio.ucsc.edu
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