What is the conventional wisdom of using a plasmid vector such as pSPORT-1
(or its sister pSPORT-2) say about transformation and cloning efficiency
when making cDNA libraries for Subtractive cloning? Can one reliably get
100,000 to a million recombinants?
Thanks in advance.
James D. Astwood E-Mail: Astwood at ccu.UManitoba.CA
Department of Plant Science Phone: (204)-474-6064
University of Manitoba FAX: (204)-261-5732
Winnipeg, Manitoba, CANADA Postal: R3T 2N2