lambda exo ssDNA sequencing

awalley at molbiol.ox.ac.uk awalley at molbiol.ox.ac.uk
Tue Aug 17 06:12:21 EST 1993


Pharmacia PCR Template Prep:

As we are having problems with direct sequencing of PCR products we are 
looking for ways to improve the template. This kit, advertised in Nature 
Genetics recently, offers the option of ssDNA sequencing using a dsDNA PCR 
product as the starting material. This is achieved by selectively degrading 
one strand using lambda exonuclease.

Does anyone have any opinions on this product or experience of it in practice 
as we hwere unablle to get a free sample from Pharmac :-)

Andrew Walley,
Paediatric Molecular Genetics,
Institute of Molecular Medicine,
Oxford, U.K.



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