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p53 mRNA (2 hybridization bands)

Ed Mccann emx1 at cdsmail.cdc.com
Mon Aug 16 20:40:14 EST 1993

In a paper by G. Strauss et.al., Int. J. Cancer 50:252(92) he described that he
observed a 4 Kb transcript for p53 beside the 2Kb band when the RNA was denature
d and electrophoresed by the formaldehyde method. However, when the RNA was dena
tured and electrophoresed by the glyoxal method mainly the 2Kb band was evident.

I also used the formaldehyde method and found 2 hybridized bands. Does anyone ha
ve any idea why the formaldehyde method gives the minor 4 Kb signal? What can th
e 4Kb message be?


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