good 5 base cutters for genomic southerns
James B. Hutchins
jbh at anat.UMSMED.EDU
Wed Aug 18 19:35:16 EST 1993
In article <1993Aug18.204951.5141 at midway.uchicago.edu>
vam2 at midway.uchicago.edu writes:
>Lastly, I'm noticing that leeches are extremely hard to grind up when I'm
>making genomic DNA. They don't homogenize very well in my 'tissuemizer'.
Haven't tried this on leeches :-* but it may work...
Wrap tissue in foil, freeze by immersion in liquid nitrogen. Store at
-80 deg C indefinitely. Pulverize in precooled mortar and pestle, then
scoop powder/goo into a container and add extraction buffer. Further
processing with the 'Tissuemizer' may be necessary but will be *much*
Hope this helps.
Jim Hutchins  E-Mail: jbh at anat.umsmed.edu
Asst Prof of Anatomy  Asst Prof of Neurology
Univ Mississippi Med Ctr  Jackson, MS
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