PEG and ligation
wmelchior at ntet.nctr.fda.gov
Wed Aug 18 09:53:45 EST 1993
Regarding the use of ligation accelerators like PEG or hexamminecobalt,
one thing about them that has always worried me is statements like the
following from Sambrook et al (page 1.70 of the second edition of
"They alter the distribution of ligation products. Intramolecular ligation
is suppressed, and the ligation products are created exclusively by
intermolecular joining events. Thus ... all the DNA products are linear
I assume this can't literally be true, or the transformation efficiency of
the ligation products would be very low, but I'd be interested in comments
on this aspect. For instance, if I want to put a blunted insert of a few
hundred bp into a plasmid of 4-5,000 bp, would I be better of using PEG or
HAC, or not?
Views expressed are not necessarily those of NCTR, its sponsoring agencies,
or the United States government.
Bill Melchior ||
National Center for Toxicological Research || ALL statements
Jefferson, AR 72079 || in this
(501) 543-7206 || signature box
|| are false.
WMELCHIOR at NTET.NCTR.FDA.GOV ||
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