Vivian Miao (vmiao at oregon.uoregon.edu) wrote:
: Has anyone had experience cutting EaeI or XmaIII (EagI) sites close to an
: end of DNA? Anyone had GOOD cutting of NOTI when there is LESS than 7
: bases between the site and the end of the DNA?
: I know about the useful references in the backs of various catalogs (e.g.
: NEB, Stratagene), and of one paper in Biotechniques, but I can't find any
: information of these enzymes specifically. [Del, Del, Del....]...
We had some good experience with doing this indirectly.
The fragments to be cut are ligated into concatamers (Fill-in if needed...).
The concatamers are then digested with your R.E. of choise, which now cuts
within the molecule with no problems. You should have enough fragments cut
Good luck, Benny.
Tel-Aviv University, Israel.
pc386 at ccsg.tau.ac.il