Run on/Run off

Brian J. Baumgartner brianb at bcm.tmc.edu
Fri Aug 20 13:09:38 EST 1993


In article <1993Aug19.092409.1 at sara.cc.utu.fi>, malaine at sara.cc.utu.fi writes:
> Hello, 
> does anybody know what is the difference between run-on and run-off
> transcription assays ?
> Thank you in advance for your answers,
> 
> Paula Mulo
> University of Turku
> SF-20500 TURKU
> Finland

Run-on refers to the transcription of genomic DNA initiated in vivo, such
as with isolated nuclei, chloroplasts, or mitochondria.  Thus the RNA
polymerase moves a short distance along the DNA but generally does not
dissociate.  Run-off refers to in vitro transcription of linear templates
in which the RNA polymerase "falls off" the end of the template.

Brian
Baylor Col. of Med.



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