I am trying to use the "Differential Display PCR" method to isolate RNA
sequences that are differentially expressed in plants under stress. I have
been reading about this method, and have had some advice from another graduate
student who does this method himself in mammalian cell samples.
I looked up a paper in Bio/Technology that described an arbitratrily primed
DNA polymorphism test using the PCR. This citation was in the Perkin-Elmer
PCR catalog under the heading "RAPD". I was surprised to see that the
detection was done by a silver staining technique instead of autoradiography.
Has anyone tried silver staining of DNA in PAGE? does it work?