genomic dna form tissue culture

Ron Garren garren at cmgm.stanford.edu
Fri Aug 20 19:56:44 EST 1993


does anyone  know a simple protocol for isolating genomic dna from
jurkat cells. I have found a method using dtap and ctap for whole
blood but i need particulars on how many cells to start with and in what
volume so I can do it all in an eppindorf.  For example I will
probably start with 1 ml or 1million cells spin down and resuspend in
300 ul( i need about 10ug). Has anyone used a protocol like this. You
can contact me at garren at cmgm.stanford.edu

		thanks ron



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