Nested Deletions

Bill Abrams wra at biochem.dental.upenn.edu
Fri Aug 20 15:55:16 EST 1993


We are using an Exonuclease III/Mung Bean Nuclease kit to generate
unidirectional deletions of double stranded DNA. A difficulty that we have
encountered is a non-linear distribution of sizes after only short
incubation times at 28 deg C. Protocols designed to generate deletions of
approximately 250 to 300 bp would be most welcome. 

Bill Abrams
wra at biochem.dental.upenn.edu



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