Silver staining DNA

Klaus.Matthaei at Klaus.Matthaei at
Sun Aug 22 04:37:29 EST 1993

>I am trying to use the "Differential Display PCR" method to isolate RNA 
>sequences that are differentially expressed in plants under stress.  I have
>been reading about this method, and have had some advice from another graduate
>student who does this method himself in mammalian cell samples.
>I looked up a paper in Bio/Technology that described an arbitratrily primed
>DNA polymorphism test using the PCR.  This citation was in the Perkin-Elmer
>PCR catalog under the heading "RAPD".  I was surprised to see that the 
>detection was done by a silver staining technique instead of autoradiography.
>Has anyone tried silver staining of DNA in PAGE?  does it work?
>Daniel Kim

Yes it does but be careful.  Under these conditions protein stains also.

Cheers, Klaus
Klaus Matthaei
Gene Targeting
The John Curtin School of Medical Research
The Australian National University
E-mail: Klaus.Matthaei at

"If at first you don't succeed - Get a bigger hammer"

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