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Enhancing nucl. acids recovery

RPSCOTT RSCOTT at IRRI.CGNET.COM
Mon Aug 23 03:19:43 EST 1993


Silicone paste was recently reported to be useful in forming a tight
junk interface during phenol-chloroform extraction in the purification of
nucleic acids. Recovery improvement of about 25% could result from the
addition of the grease into the extraction mixture. I just discovered the
report a few days ago and would try it soon but it seems quite promising.
I know others who haven't heard of it yet could find it useful too.

	MUKHOPADHAY, T. & ROTH, J. [U. of Texas] (1993) Silicone
	lubricant enhances recovery of nucleic acids after phenol-
	chloroform extraction.

I hope other netters who have already tried this out could share their 
experiences to the group.

Scott

======================================
R. Scott
Division of Plant Pathology
International Rice Research Institute
POB 933, 1099 Manila, Philippines
e-mail: rscott at irri.cgnet.com
======================================




 




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