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Enhancing nucl. acids recovery

Mon Aug 23 03:19:43 EST 1993

Silicone paste was recently reported to be useful in forming a tight
junk interface during phenol-chloroform extraction in the purification of
nucleic acids. Recovery improvement of about 25% could result from the
addition of the grease into the extraction mixture. I just discovered the
report a few days ago and would try it soon but it seems quite promising.
I know others who haven't heard of it yet could find it useful too.

	MUKHOPADHAY, T. & ROTH, J. [U. of Texas] (1993) Silicone
	lubricant enhances recovery of nucleic acids after phenol-
	chloroform extraction.

I hope other netters who have already tried this out could share their 
experiences to the group.


R. Scott
Division of Plant Pathology
International Rice Research Institute
POB 933, 1099 Manila, Philippines
e-mail: rscott at irri.cgnet.com


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