> From: IN%"<@CGNET.COM:dkim at unm.edu>" "dkim" 21-AUG-1993 03:43
>> I looked up a paper in Bio/Technology that described an arbitratrily primed
> DNA polymorphism test using the PCR. This citation was in the Perkin-Elmer
> PCR catalog under the heading "RAPD". I was surprised to see that the
> detection was done by a silver staining technique instead of autoradiography.
>> ...Daniel Kim
Silver staining affords crisper resolution of closely-spaced bands
in polyacrylamide gels than isotopic detection although sensitivity remains
We perform a lot of RAPD experiments here but we generally resort to
agarose-based fractionation of PCR products. Then we just ethidium-stain our
gels. We get excellent separation of 0.5-3 kb fragments on 0.50% agarose-
0.75% synergel composite in TBE.
For silver-staining the Guilemette-Lewis technique is recommended (Molec.
Genet. Anal. of Populations (1992), ed. by A.R. Hoelzel (IRL Press)):
Guillemette, J. & Lewis, P. (1983) Electrophoresis 4:92.
Division of Plant Pathology
International Rice Research Institute
POB 933, 1099 Manila, Philippines
e-mail: rscott at irri.cgnet.com