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Silver staining DNA

Mon Aug 23 03:19:02 EST 1993

> From:	IN%"<@CGNET.COM:dkim at unm.edu>"  "dkim"    21-AUG-1993 03:43
> I looked up a paper in Bio/Technology that described an arbitratrily primed
> DNA polymorphism test using the PCR.  This citation was in the Perkin-Elmer
> PCR catalog under the heading "RAPD".  I was surprised to see that the 
> detection was done by a silver staining technique instead of autoradiography.
> ...Daniel Kim

Silver staining affords crisper resolution of closely-spaced bands
in polyacrylamide gels than isotopic detection although sensitivity remains

We perform a lot of RAPD experiments here but we generally resort to
agarose-based fractionation of PCR products. Then we just ethidium-stain our
gels. We get excellent separation of 0.5-3 kb fragments on 0.50% agarose-
0.75% synergel composite in TBE.

For silver-staining the Guilemette-Lewis technique is recommended (Molec.
Genet. Anal. of Populations (1992), ed. by A.R. Hoelzel (IRL Press)):

    Guillemette, J. & Lewis, P. (1983) Electrophoresis 4:92.

Genetics Laboratory
Division of Plant Pathology
International Rice Research Institute 
POB 933, 1099 Manila, Philippines
e-mail: rscott at irri.cgnet.com


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