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Enhancing nucl. acids recovery

srgcles at grv.grace.cri.nz srgcles at grv.grace.cri.nz
Mon Aug 23 16:09:02 EST 1993


In Article <01H21TLBRJUO00027T at irri.cgnet.com>
RSCOTT at IRRI.CGNET.COM (RPSCOTT) writes:
>Silicone paste was recently reported to be useful in forming a tight
>junk interface during phenol-chloroform extraction in the purification of
>nucleic acids. Recovery improvement of about 25% could result from the
>addition of the grease into the extraction mixture. I just discovered the
>report a few days ago and would try it soon but it seems quite promising.
>I know others who haven't heard of it yet could find it useful too.
>
>	MUKHOPADHAY, T. & ROTH, J. [U. of Texas] (1993) Silicone
>	lubricant enhances recovery of nucleic acids after phenol-
>	chloroform extraction.
>
Thanks, this looks interesting and I'd like to give it a go. Could you please 
post a full reference to allow me to get the paper? Thanks for your help and 
input on the net.

Brian Scrimshaw
Institute of Environmental Health and Forensic Sciences
PO Box 30547
>Lower Hutt
Wellington
New Zealand



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