What is the copy number of pTrc99?
txt15 at po.CWRU.Edu
Mon Aug 23 14:15:59 EST 1993
For subcloning purposes, I have to use a plasmid with polycloning sites
that has relatively lower copy number than the pUC based plasmid. One I
am interested in using is the pTrc99 from Pharmacia. I have no idea on
the exact copy number of this plasmid, that's why I am asking you for
TIA for any reply.
p.s. For someone working on Salmonella, it seems to me that pUC based
plasmid is extremely unstable in this bug. Anybody have similar
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