What is the copy number of pTrc99?

Tao Tao txt15 at po.CWRU.Edu
Mon Aug 23 14:15:59 EST 1993


Dear Netters:

For subcloning purposes, I have to use a plasmid with polycloning sites
that has relatively lower copy number than the pUC based plasmid.  One I
am interested in using is the pTrc99 from Pharmacia.  I have no idea on 
the exact copy number of this plasmid, that's why I am asking you for
help.

TIA for any reply.

Tao

p.s.  For someone working on Salmonella, it seems to me that pUC based
	plasmid is extremely unstable in this bug.  Anybody have similar
	experience?
-- 
p



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