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What is the copy number of pTrc99?

Tao Tao txt15 at po.CWRU.Edu
Mon Aug 23 14:15:59 EST 1993

Dear Netters:

For subcloning purposes, I have to use a plasmid with polycloning sites
that has relatively lower copy number than the pUC based plasmid.  One I
am interested in using is the pTrc99 from Pharmacia.  I have no idea on 
the exact copy number of this plasmid, that's why I am asking you for

TIA for any reply.


p.s.  For someone working on Salmonella, it seems to me that pUC based
	plasmid is extremely unstable in this bug.  Anybody have similar

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