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Protein blotting to PVDF membranes

David Martin x3175 dmartin at crc.ac.uk
Mon Aug 23 09:11:04 EST 1993

We routinely blot to PVDF but use 10mM CAPS pH 11 10% MeOH as the transfer
buffer in a mini protean cell at a current of 100 mA. It takes ca. 60-90

to stain, 30 secs in coomassie (1g/l 40% MeOH 10% HOAc) then destain in
40% MeOH 10% HOAc. repeat to enhance sensitivity (maybe use a 15 or 20 sec

we use ABI ProBlott.. and use the protocol in the instructions. Our peptide
chemist reckons if you can see the band, it'll sequence (unless it is 

good luck

...david Martin

dmartin at uk.ac.crc

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