PMN DNA isolation

herro001 at maroon.tc.umn.edu herro001 at maroon.tc.umn.edu
Tue Aug 24 15:12:35 EST 1993


I have been isolating DNA by chloroform/phenol extraction from human
neutrophils.  After isolation I have been running the isolated preps on 1%
agarose gels.  I have found that very little DNA shows up in the gels
(by ETBr) when I use fresh cells.  If I let the cells sit in the incubator
for 2 or 3 hours more dna shows up in the gels (more and more dna shoes up
for the first 12 hours).

Is this "normal"?
Can someone explain or guess what is happening?

Mike Herron
herro001 at maroon.tc.umn.edu



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