PCR of 3 kbp fragments from genomic DNA

rjb at canctr.mc.duke.edu rjb at canctr.mc.duke.edu
Tue Aug 24 08:10:04 EST 1993


In article <930823153417.20203974 at FCRFV2.NCIFCRF.GOV>, FORNACE at FCRFV2.NCIFCRF.GOV writes:
>Dear Netters:
>Is there a FAQ on methods to successfully PCR large
>DNA fragments from mammalian DNA, or could anyone with
>suggestions respond directly to me?
>Thanks!
>
>Al Fornace    email:   fornace at ncifcrf.gov

Try HotTub Polymerase - I heard it's good for amplification of
larger fragments.
Good Luck
Rohan de Silva



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