Protein blotting to PVDF membranes

KARN at Butler.EDU KARN at Butler.EDU
Tue Aug 24 07:38:57 EST 1993


In regard to the concern expressed about exposing proteins to acetic acid,
to wit- won'
won't it block the N-terminal end?  Note that acylation of N-termini normally
occurs via an enzymatic mechanism and can involve any number of different
sized groups from formyl on up.  Not only does exposure to organic acids
not block proteins, use of such acids as media for solubilizing peptides in
the course of their separation and purification is classical protein chemistry.
For reference, see Needleman's books on protein chemistry.
Bob Karn
KARN at BUTLERU.EDU



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