Protein blotting to PVDF membranes

David Martin x3175 dmartin at crc.ac.uk
Tue Aug 24 03:00:14 EST 1993


Potentially yes, it may acylate. But no one has told the acetic acid or the 
protein yet so whilst they exist in blissful ignorance we still get good 
sequence.

We run the sds gels with thioglycolate as well. I haven't had any problems
the times I forget to add the thioglycolate.

This set up works in our hands quite well.. 

..d



More information about the Methods mailing list