MacConkeys instead of Xgal/IPTG selection?

Robert Rumpf rrumpf at magnus.acs.ohio-state.edu
Thu Aug 26 09:22:28 EST 1993


I've heard that the alpha-complementation-based color selection using 
lacZ-phagemids, lacZ deletion host cells, and Xgal/IPTG plates can also be done
using the same phagemids/host cells but plated onto MacConkey's agar.  Is this 
true, how is it done, and what's the mechanism for color selection?  I 
appreciate any help you can give...



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