Lambda screening

Thomas_C.Newman 22313TCN at MSU.EDU
Thu Aug 26 09:10:00 EST 1993


Fellow netters:

  As part of a sequencing project we have produced a large number of
microtiter plates which contain our initial lambda plaque picks (1 plaque in
400 ul). My question is whether anyone has ever directly replicated from
microtiter plates to nitrocellulose for subsequent probing?  Is there
sufficient material to get a good signal, and how was the protein coat removed
from the phage?
  Thanks in advance.  You may email directly to me at: 22313tcn at msu.edu.

Tom Newman



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