PCR - amplification

[TEMANUL at DUCVAX.AUBURN.EDU]

Hi,
I will try to amplify a 2 kb genomic DNA fragment using 25mer primers
(mp= 60 C, 100 % homology) Has anybody experience and can give me 
optimal or standard conditions for the PCR (DNA/primer concentration,
reaction temperatures/time) to start? Thanks, Gela.