PCR - amplification

TEMANUL at DUCVAX.AUBURN.EDU TEMANUL at DUCVAX.AUBURN.EDU
Thu Aug 26 09:28:00 EST 1993


Hi,
I will try to amplify a 2 kb genomic DNA fragment using 25mer primers
(mp= 60 C, 100 % homology) Has anybody experience and can give me 
optimal or standard conditions for the PCR (DNA/primer concentration,
reaction temperatures/time) to start? Thanks, Gela.



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