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PCR - amplification

Paul N Hengen pnh at fcsparc6.ncifcrf.gov
Thu Aug 26 15:22:48 EST 1993

In article <01H270UZ8NJK002FUD at DUCVAX.AUBURN.EDU>

> I will try to amplify a 2 kb genomic DNA fragment using 25mer primers
> (mp= 60 C, 100 % homology) Has anybody experience and can give me 
> optimal or standard conditions for the PCR (DNA/primer concentration,
> reaction temperatures/time) to start? Thanks, Gela.

I've mailed you the FAQ list which is a good place to start.
In there you will find a list of good reviews and books for
optimizing your PCR reactions.

Paul N. Hengen
National Cancer Institute
Frederick Cancer Research and Development Center
Frederick, Maryland 21702-1201 USA

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