PCR cloning and Xgal
MYGALP01 at ULKYVM.LOUISVILLE.EDU
Thu Aug 26 15:15:59 EST 1993
There were several reports that pUC with cloned 0.6 kb PCR fragments
can still give light blue color on Xgal plates. Did anybody have the same
experience with longer fragments? Alternatively, has anybody seen short
in-frame fragments giving clear colonies?
You can send answers directly to me. If there is sufficient interest,
I'll post the summary.
Michael Galperin, pH.D. Bitnet: mygalp01 at ulkyvm
U of L - Microbiology Voice: (502) 588-7539
Louisville, KY 40292 Fax: (502) 588-7531
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