PCR cloning and Xgal

Michael Galperin MYGALP01 at ULKYVM.LOUISVILLE.EDU
Thu Aug 26 15:15:59 EST 1993


    Hi, netters!
    There were several reports that pUC with cloned 0.6 kb PCR fragments
 can still give light blue color on Xgal plates. Did anybody have the same
 experience with longer fragments?  Alternatively, has anybody seen short
 in-frame fragments giving clear colonies?
    You can send answers directly to me. If there is sufficient interest,
 I'll post the summary.

Michael Galperin, pH.D.                 Bitnet: mygalp01 at ulkyvm
U of L - Microbiology                    Voice: (502) 588-7539
Louisville, KY  40292                      Fax: (502) 588-7531



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