Any body have a good protocol for preparing primers after synthesis. We have
an ABI DNA synthesizer which we use to make our primers. We deprotect them
by incubating at 55c for 5 hours then dry in a savant spinvac. My questions:
1) Is there any way to speed up the deprotect process?
2) After I dry my primers there's always a bunch of crud at the booton of
the tube that never solubilizes when I add h20. Is this wasted oligomer? Is
there a way to avoid this?
Any insights will help, thanks...
Cooper
Picard at helix.nih.gov
