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Lambda ExCell vector

TIMO KERVINEN MMKAB_OPISK tkervine at viikki.Helsinki.FI
Mon Aug 30 04:54:57 EST 1993


Hi all!

I am wondering if someone have experience using Lambda ExCell cloning vector
by Pharmacia in the construction of cDNA library. I would like to know how
it has worked in the following techniques: ligation of cDNA, in vitro 
packaging of recombinant vector (vendor of packaging extract), in vivo 
excision by E.coli NP66 and sequencing in pExCell (what primers etc.).  

Thank you in advance

Timo Kervinen
Department of Plant Pathology
University of Helsinki
Finland



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