avidin-biotin separation

ZUNINO at PLAUTO.CSATA.IT ZUNINO at PLAUTO.CSATA.IT
Tue Aug 31 11:43:00 EST 1993


Dear netters,
we are trying to purify some biotinylated DNA fragments with avidin-sepharose
or strepavidin-sepharose beads. However, since we want to clone this DNA
we have to detach the avidin from the biotin to recover free DNA molecules.
The avidin-biotin interactions are extremely strong and we do not know of a
good means to destroy such interactions. We do not have now the Biotin-UTP
containing an S-S bridge, which would be easy to cut with a reducing agent.
So, we wonder if there is anyone there that knows how we could separate 
free DNA fragments after having recovered the biotinylated DNA. 
Thanks to all.
M. Binaschi
Istituto Nazionale Tumori
20133 Milan, Italy
zunino at mvx36.csata.it



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