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avidin-biotin separation

Drew Smith drsmith at shaman.nexagen.com
Tue Aug 31 12:33:21 EST 1993


>Dear netters,
>we are trying to purify some biotinylated DNA fragments with avidin-sepharose

>M. Binaschi
>Istituto Nazionale Tumori
>20133 Milan, Italy
>zunino at mvx36.csata.it

Pierce sells immobilized monomeric avidin (cat#20228, $205/5ml gel).  Its 
Kd with biotin is in the nM range.  You elute off the bound biotin
conjugate with free biotin, under native conditions.  The gel can then
be regenerated by washing at pH2

Drew Smith
drsmith at nexagen.com

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