minigels on slides keep floating away - help

donald seto don at helix.nih.gov
Tue Aug 31 14:49:54 EST 1993


In article <9308242344.AA16474 at deimos.med.pitt.edu> bsh at MED.PITT.EDU (Basavaraju Shankarappa) writes:
>> Shiver me timbers! I have been making little mini-gels on frosted
>> microscope slides, by mixing molten 1% LMP agarose with lymphocytes, and
>> pipeting this onto the slides, and overlaying the agarose with a
>> coverslip. Later, I remove the coverslip (carefully sliding it off the
>> agarose) and place the slide in an alkaline lysis buffer. However, many
>> times my gels will separate from the slides. Any sugesstions? Thanks, Greg.
>> 
>To steal an idea from the BRL, in their Horizon electrophoresis apparatus
>they have small depressions at four corners so that the gel will hold after
>pouring in.  I guess you can improvise on this either by making small
>depressions on the slide's surface or add a few microliters of wax or
>some other resin like superglue.  These raised surfaces will hold the 
>gel in place for most manipulations.

to borrow an idea from immunochemists, you could also spot the slides with
some agarose, let this airdry, till it is flat with the slide, and then
pour your gel mixture on top of that....the gel sticks very tightly.
--
********************************************************************
                         don at helix.nih.gov
301-402-0580   Bldg10/Rm8D14    NIDDK/NIH       Bethesda, MD  20892



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