X-gal Staining Blues -- Why Ain't My Cells Gettin' Blue?

Martin Leach leach at mbcrr.harvard.edu
Wed Dec 1 17:08:37 EST 1993


In article <CHC17A.792 at ncifcrf.gov>, masuda at fcs280b.ncifcrf.gov (Michiaki
Masuda) wrote:
> 
>    Essentially, I didn't see any difference between the transfected NIH3T3
> cells and the untransfected control. Although the possibility cannot be
> excluded completely that there is something wrong with the plasmid or
> the transfection procedure, results of other experiments sugget that the
> staining procedure is the prime suspect.
> 
>    I'd appreciate it if someone could provide me with his/her protocol
> working successfully and/or some secret tips to help me out from...

Dear netter,

do you know that the DNA is getting into the cells???

have you done a b-gal assay on extracts to show this???

sounds like the DNA isn't getting into the cells.....


Martin Leach

-- 

.....          Martin Leach                Email:leach at mbcrr.harvard.edu 
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